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Graham is also one of the few living products of artificial insemination experiments in red wolves, a technique that Will wants to further hone. Artificial insemination may be useful to the program in the future in case the captive breeding efforts to create matched pairs based on mean kinship falls short of reproductive goals. In some cases, the captive female wolves were so aggressive to males that they were paired with that breeding never took place. Artificial insemination and securing red wolf sperm from all of the founding lines of the captive breeding program were identified in the 1990 Red Wolf Recovery/Species Survival Plan. Not long after, workers began collecting red wolf sperm. According to Will, there happened to be a fellow outside of Portland who had an international canid semen bank. The captive breeding program contracted him to help explore the viability of using electroejaculation on wolves. In the 1990s, Will helped develop protocols for immobilizing, catheterizing, and stimulating the wolves they sampled. They went through the captive population and methodically collected and evaluated sperm from male red wolves. If the sperm met certain criteria, it was processed and frozen. Eventually, the cryopreserved red wolf semen bank held samples from sixty individuals, which collectively represented thirteen of the fourteen founding lines. The work died down when a grant ran out and one of the main reproductive physiologists switched jobs.
Today, the early efforts to cryopreserve red wolf sperm and artificially inseminate females have proven their value - not just through Graham and his antics at the exhibit, but because a wolf named Stubs, one of the last living representatives from one of the fourteen founding lines, died in 1998. With Stubs’s death, the unique diversity of his founding lineage died within the captive population. However, his semen was collected and banked before he died. Will would like nothing more than to resurrect Stubs’s line. The only challenge, he says, is that the previous two cases of artificial insemination, in 1992 and 2003, were done using fresh sperm. In the first case, a six-year-old red wolf birthed two females and one male. Graham was born into a different litter of five.
“Clearly, we know artificial insemination using cryopreserved sperm works. It’s been done in other animals,” Will says. “We just need to pick this research up again and see where it might lead.” If male red wolves experience a decrease in sperm quality, or if females are behaviorally uncooperative with their male counterparts, then artificial insemination may move from the realm of research to that of necessity. It’s always nice to have a backup plan.
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T. DeLene Beeland (The Secret World of Red Wolves: The Fight to Save North America's Other Wolf)